Yukiko Amagi is a high school second year and the daughter of the family who runs the venerable, high-class Amagi Inn. She spends a lot of time with Chie Satonaka, and is currently in the middle of her apprenticeship as manager.Yukiko's initial persona is Konohana Sakuya, and her evolved persona is Amaterasu, both of which specialize in fire and healing skills.
Yukiko creates the Priestess Arcana Social Link with the protagonist, and through the protagonist's interactions with her, Yukiko expresses how she cannot live a normal teenager's life due to her status as the Amagi heir, and her wish to leave Yasoinaba instead of inheriting the Inn. She decides to become an interior decorator after her graduation. During their interactions, however, Yukiko also reveals that she is constantly being pestered by tabloid reporters after the murder of Mayumi Yamano, the first murder victim and TV announcer, who lived in her Inn before being murdered.
Yukiko also confesses her horrible cooking skills, and is worried that she would not be able to take care of herself after leaving the town. She asks the protagonist to sample her food during their interactions. Yukiko later enlists the Amagi Inn's cook to teach her, under the excuse of doing it for a boy. As the protagonist interacts with Yukiko, Yukiko slowly loses her urge to leave the town. She eventually realizes how much she loves the Inn after being harassed by the tabloid reporters, who want to publish a story that would tarnish the Inn's reputation. Finally having had enough, Yukiko angrily threatens them away.
By the end of the Social Link, Yukiko decides not to leave Yasoinaba, but to inherit the Inn and continue her family's legacy. Having finally found her resolution, Yukiko's Persona, Konohana Sakuya transfigures into Amaterasu.
Should the protagonist chooses to establish a lovers relationship with Yukiko and accepts her invitation in Dec 24, the protagonist bakes Yukiko a Christmas Cake, and she then gives him a Leather Keyring that guards against Fire-elemental attacks. Yukiko will also ask the protagonist if she can stay over for the night, saying that she told her parents that she is staying in Chie's house for the night.
In the events of the Chariot Arcana Social Link, Chie reveals that she first met Yukiko during their childhood. The day that they met, Yukiko ran away from home with a dog. According to Chie, Yukiko was expressionless as a child, and reveals that Yukiko ran away from home because her family did not allow her to take in the stray dog. Feeling sympathetic towards Yukiko, Chie tries to make Yukiko smile, and the two eventually become best friends. Chie later reveals that the dog became her pet.